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Location: Irvine, California
Ships to: US,
Item: 286159656977
Restocking Fee:No
Return shipping will be paid by:Buyer
All returns accepted:Returns Accepted
Item must be returned within:30 Days
Refund will be given as:Money Back
Applied Biosystems:Applied Biosystems
MPN:4306737
Material:Polystyrene
Country/Region of Manufacture:China
The MicroAmp Optical 96-Well Reaction Plate with Barcode is a PCR reaction plate designed specifically for use in real-time PCR (qPCR) and other optical-based assays. It is a popular choice for laboratories conducting high-throughput analysis, particularly in applications requiring precise detection and quantification of nucleic acids. Here are the key features and details of this plate:Key Features:96-Well Format: The plate contains 96 individual wells, commonly used for high-throughput applications. This format is compatible with standard 96-well PCR systems and qPCR instruments.Optical Quality: The plate is designed for optical detection, meaning it is made from materials that allow for optimal fluorescence emission and detection during PCR amplification. This makes it ideal for quantitative PCR (qPCR) and other optical detection assays, such as DNA sequencing, genotyping, or gene expression analysis.Barcode: Each plate comes with a barcode for easy sample tracking and identification, which is particularly useful in high-throughput environments where many samples are being processed simultaneously. The barcode can help automate workflows and minimize human error during sample handling.Material: Typically made from polypropylene or a similar durable polymer, the material is chosen for its ability to withstand the thermal cycling conditions of PCR and for its chemical resistance to commonly used reagents in PCR and qPCR reactions.Thermal Performance: The design of the plate allows for consistent and uniform thermal cycling, crucial for accurate PCR amplification. The material and construction help to ensure even heat distribution during the amplification process.Well Volume: The wells are designed to accommodate standard PCR reaction volumes, typically in the range of 10-50 µL, depending on the assay requirements.Compatible with Seals: The plate is compatible with various sealing options, such as optical adhesive seals, optical caps, and foil seals, to prevent evaporation and contamination during thermal cycling.Applications:Real-Time PCR (qPCR): The MicroAmp Optical 96-Well Reaction Plate is ideal for quantitative PCR applications, where the goal is to amplify and quantify DNA or RNA sequences based on fluorescence measurements.Gene Expression Analysis: It is used for studying gene expression patterns by measuring the amplification of specific gene targets, typically through the use of fluorescent probes or dyes.Genotyping: The plate is often used in genotyping assays to detect genetic variations, such as single nucleotide polymorphisms (SNPs) or mutations.Next-Generation Sequencing (NGS): It is also used in workflows where PCR is a step before sequencing or for preparing samples for sequencing.High-Throughput Screening: With its 96-well format, it is suitable for large-scale sample testing in research and diagnostic laboratories, such as drug discovery, biomarker validation, and clinical diagnostics.Key Benefits:High Throughput: The 96-well format allows for the simultaneous processing of many samples, which is essential for large-scale assays and studies.Barcode for Automation: The barcode system enhances workflow automation, sample identification, and reduces manual handling errors.Optical Compatibility: Designed specifically for real-time PCR and optical detection, ensuring accurate and reliable fluorescence-based measurements.Ease of Use: The plate is easy to handle, and its design is compatible with most major PCR instruments, including those from Applied Biosystems, Thermo Fisher, and others.Seal Compatibility: The ability to use various sealing options (optical seals) helps prevent evaporation and contamination, making it suitable for long-duration runs and sensitive assays.Packaging:The MicroAmp Optical 96-Well Reaction Plate with Barcode is usually sold in packs, often with 10 plates per box, although this can vary depending on the supplier.Considerations:Not Suitable for Cell Culture: As an optical PCR plate, it is not designed for cell culture or assays that require adherent cells.Optical Sensitivity: If used in fluorescence-based assays, be sure the plate is compatible with the fluorophores or dyes used in your experiments, as some plates have better optical properties than others.Summary: The MicroAmp Optical 96-Well Reaction Plate with Barcode is a highly specialized consumable designed to provide accurate and reliable results in real-time PCR and other fluorescence-based assays. Its optical clarity, compatibility with seals, and high-throughput design make it a popular choice for molecular biology, genetic research, diagnostics, and other applications requiring precise DNA amplification and quantification.
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